Nucleosomal H2B ubiquitylation with purified factors
نویسندگان
چکیده
منابع مشابه
The role of H2B ubiquitylation in development
The formation of a multi-cellular organism requires co-ordinated rounds of cellular proliferation, differentiation and apoptosis, to generate, specify and shape body tissues. Strict regulation of proliferation and apoptosis are important for preventing uncontrolled cell growth, the hallmark of cancer. Meanwhile, spatial and temporal control of gene expression gives rise to a myriad of specializ...
متن کاملRegulation of histone H2A and H2B ubiquitylation.
Histone ubiquitylation has emerged as an important chromatin modification with roles in transcription and trans-histone methylation. In the past several years, there has been dramatic progress in the identification of factors that control ubiquitin attachment to the core histones H2A and H2B. Recent advances in this area will be reviewed, and outstanding questions relating to the cellular funct...
متن کاملHistone H2B ubiquitylation is associated with elongating RNA polymerase II.
Rad6-mediated ubiquitylation of histone H2B at lysine 123 has been linked to transcriptional activation and the regulation of lysine methylation on histone H3. However, how Rad6 and H2B ubiquitylation contribute to the transcription and histone methylation processes is poorly understood. Here, we show that the Paf1 transcription elongation complex and the E3 ligase for Rad6, Bre1, mediate an as...
متن کاملHistone H2B ubiquitylation disrupts local and higher order chromatin compaction
Regulation of chromatin structure involves histone posttranslational modifications that can modulate intrinsic properties of the chromatin fiber to change the chromatin state. We used chemically defined nucleosome arrays to demonstrate that H2B ubiquitylation (uH2B), a modification associated with transcription, interferes with chromatin compaction and leads to an open and biochemically accessi...
متن کاملBre1p-mediated histone H2B ubiquitylation regulates apoptosis in Saccharomyces cerevisiae.
BRE1 encodes an E3 ubiquitin protein ligase that is required for the ubiquitylation of histone H2B at lysine 123 (K123). Ubiquitylation of this histone residue is involved in a variety of cellular processes including gene activation and gene silencing. Abolishing histone H2B ubiquitylation also confers X-ray sensitivity and abrogates checkpoint activation after DNA damage. Here we show that Sac...
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ژورنال
عنوان ژورنال: Methods
سال: 2011
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2011.03.009